Direct evidence of surface infestation of seeds and tubers by Plasmodiophora brassicae and quantification of spore loads

Using quantitative PCR, DNA of Plasmodiophora brassicae, the causal agent of clubroot, was detected and quantified on canola, pea and wheat seeds, as well as on potato tubers, all harvested from clubroot‐infested fields in Alberta, Canada. Quantifiable levels of infestation were found on seven of the 46 samples analysed, and ranged from <1·0 × 10³ to 3·4 × 10⁴ resting spores per 10 g seeds; the vast majority (80–100%) of resting spores on these samples were viable, as determined by Evan’s blue vital staining. However, the levels of infestation found were generally lower than that required to cause consistent clubroot symptoms in greenhouse plant bioassays. While the occurrence of P. brassicae resting spores on seeds and tubers harvested from clubroot‐infested fields suggests that seedborne dissemination of this pathogen is possible, practices such as commercial seed cleaning may be sufficient to effectively mitigate this risk.     

太白山林木真菌病害的垂直分布

我们对秦岭主峰太白山的林木真菌病害的分布规律进行了多年的探讨,结果表明,太白山林木真菌病害可划分为四个垂直分布带。即低山、低中山阔叶林叶点霉病和白粉病分布带;中山、高中山阔叶林锈病和立木腐朽分布带;中高山针叶林散斑病分布带;高山灌丛漆斑病分布带。     

Dielectric properties of copper-ethanolamine treated Chinese fir (Cunninghamia lanceolata Hook.)

In order to clarify the interaction between copper and wood substances in wood treated with copper containing water-borne wood preservatives, the dielectric constant ε′ and dielectric loss factor ε″ of untreated wood and wood treated with four concentration levels of copper-ethanolamine (Cu-EA) solutions were determined within a temperature range from –100 to 40°C and a frequency range from 100 to 1 MHz. Three dielectric relaxation processes were observed in the ε″ spectrum; among them R-I is based on the reorientation of methylol groups in the amorphous region of wood cell walls and R-II is related to wood extractives. R-III appeared in Cu-EA treated wood, and its magnitude decreases with the concentration of Cu-EA solutions used in this experiment. This relaxation process was considered to be based on the reorientation of copper-ethanolamine-wood complexes in wood cell walls. At low copper retention, the hydrogen in the complex can form hydrogen bonding with adjacent hydroxyl groups, which results in a strong bonding state between copper and wood; at high copper retention, the numerous copper-ethanolamine complexes not only hinder them from forming hydrogen bonding with adjacent wood molecules due to steric hindrance, but also weaken the interaction between wood molecules themselves, which corresponds to reducing ε″ values of both R-I and R-III processes. The results explain the fact of in-creasing copper leaching in wood treated with high concentration copper-based water-borne preservatives.     

茶秆竹生物量模型研究

在标准地调查的基础上，应用多种数学模型对茶秆竹各器官生物量进行相关性分析，建立了茶秆竹各器官间的咽归模型。可用于一定立地条件下的茶秆竹的生物量估测。     

银杏种子后熟生理与内源激素变化的研究

研究银杏种子后熟期间的生理变化以及内源激素对种子休眠与萌发过程的调控作用.结果表明:银杏种胚分化不完全、发育不充分是种子休眠的主要原因,银杏种子的后熟过程实质上是种胚不断分化生长的过程.在此过程中,物质代谢、酶活性的变化均有利于种胚发育和种子发芽,种子内部经历了一系列生理生化变化去调整内源激素平衡、完成种胚发育.研究发现,内源激素在银杏种子休眠与萌发过程中起着重要的作用:GA1 3是银杏种胚后熟的关键物质;ZRs对银杏种子生长起重要的促进作用;GA1 3/ABA及ZRs/ABA相对比例的变化对种子发育、胚的后熟及种子萌发均有重要的影响.     

油茶根腐病发生规律和综合防治研究

油茶根腐病是油茶林的新病害，植株自发病至死亡为２～３年，其病菌为根际土壤习居菌，喜酸性和较低温度，病菌侵入植株的途径主要是伤口，潜育期２０ｄ左右．防治本病的关键是保护幼树根颈部及主根不受伤害，加强管理，增强树势．此外，用熟石灰在根际处与表土层拌匀覆盖和用多菌灵、退菌特等杀菌剂及时进行防治，并辅以营林技术措施治理，可收到良好的防治效果．     

浅论济南可持续发展的道路景观设计

城市道路不仅具有交通功能,是展示城市形象的重要窗口。该文以济南市为例,分析了城市道路景观的现状及存在的一些问题,结合自然条件、历史人文、环境生态等要素,阐述了可持续发展的城市道路景观设计内容,简述了"社会、经济、自然"三位一体的可持续发展的道路景观设计方向。     

Relationship between photosynthesis and leaf nitrogen concentration in ambient and elevated [CO2] in white birch seedlings

To study the effects of elevated CO2 concentration ([CO2]) on relationships between nitrogen (N) nutrition and foliar gas exchange parameters, white birch (Betula papyrifera Marsh.) seedlings were exposed to one of five N-supply regimes (10, 80, 150, 220, 290 mg N l(-1)) in either ambient [CO2] (360 micromol mol(-1)) or elevated [CO2] (720 micromol mol(-1)) in environment-controlled greenhouses. Foliar gas exchange and chlorophyll fluorescence were measured after 60 and 80 days of treatment. Photosynthesis showed a substantial down-regulation (up to 57%) in response to elevated [CO2] and the magnitude of the down-regulation generally decreased exponentially with increasing leaf N concentration. When measured at the growth [CO2], elevated [CO2] increased the overall rate of photosynthesis (P(n)) and instantaneous water-use efficiency (IWUE) by up to 69 and 236%, respectively, but decreased transpiration (E) and stomatal conductance (g(s)) in all N treatments. However, the degree of stimulation of photosynthesis by elevated [CO2] decreased as photosynthetic down-regulation increased from 60 days to 80 days of treatment. Elevated [CO2] significantly increased total photosynthetic electron transport in all N treatments at 60 days of treatment, but the effect was insignificant after 80 days of treatment. Both P(n) and IWUE generally increased with increasing leaf N concentration except at very high leaf N concentrations, where both P(n) and IWUE declined. The relationships of P(n) and IWUE with leaf N concentration were modeled with both a linear regression and a second-order polynomial function. Elevated [CO2] significantly and substantially increased the slope of the linear regression for IWUE, but had no significant effect on the slope for P(n). The optimal leaf N concentration for P(n) and IWUE derived from the polynomial function did not differ between the CO2 treatments when leaf N was expressed on a leaf area basis. However, the mass-based optimal leaf N concentration for P(n) was much lower in seedlings in elevated [CO2] than in ambient [CO2] (31.88 versus 37.00 mg g(-1)). Elevated [CO2] generally decreased mass-based leaf N concentration but had no significant effect on area-based leaf N concentration; however, maximum N concentration per unit leaf area was greater in elevated [CO2] than in ambient [CO2] (1.913 versus 1.547 g N m(-2)).     

皂荚(属)研究进展及产业化发展前景*

综述了国内外皂荚(属)主要研究进展,包括引种栽培、种实、刺特征与成份、抗逆性、药用价值、生态经济价值、食品及其它工业的开发应用、繁殖技术、遗传学研究等方面。从皂荚(属)资源保护、评价与合理利用及产业化发展的角度,提出开展种源试验与选择、繁殖技术研究,建立良种选育机制和种质资源保存机制,完善加工型企业,从而促进社会经济增长等产业化发展对策。     

臭椿、新疆杨混交林对光肩星天牛选择寄主行为的影响

利用3年生臭椿、新疆杨在室内建立混交林进行光肩星天牛成虫放飞试验.结果表明:臭椿、新疆杨混交林对天牛成虫的寄主选择有显著影响,天牛成虫的交尾行为只在新疆杨植株上进行;天牛成虫在林内植株上着落的总虫次随新疆杨株数的减少而下降,交尾次数也减少;臭椿、新疆杨植株数量在林分中所占比例在一定范围时,天牛成虫在新疆杨和臭椿上着落的虫次比均趋于稳定;新疆杨与臭椿以行状3:2和2:3混交时林分的抗虫效果较好.